I spent my lunch break testing that, in fact. I first booted into 7.1 at 44.44MHz, verified AppleTalk was off (in the Chooser), then rebooted into 7.6.1, which was fine. I confirmed AppleTalk was off there too, then shutdown, pulled the motherboard, flipped the switch on Spicy O'Click to the Max setting (49.16MHz), then rebooted. 7.6.1 froze during the boot sequence. Thankfully, I was able to reboot with Shift held down which got me to the Desktop. That doesn't always work though. I verified AppleTalk was off in 7.6.1, then used System Picker to reboot into OS 8.1. That booted (not surprising, as it randomly boots and doesn't boot). I verified it was OFF in 8.1 too. But as soon as I start changing Control Panels or Extensions (even if but to enable and then disable only one), I often get a freeze during the boot sequence, at the very beginning where the bar graph just starts to show. It freezes before any CP icons appear at the bottom.
So AppleTalk being ON or OFF doesn't seem to matter. At 44.44MHz, all 3 OS's work fine. At 49.16MHz, only 7.1 and 7.5.5 reliably boot, with 7.6.1 & 8.1 being hit and miss. And again, sometimes even rebooting with Shift held down won't stop the freeze. I then either need to boot from a floppy or pull the motherboard to kick down the clock speed.
Still looking forward to your testing of these OS's,
@Drake !